verso cdna kit (Thermo Fisher)

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Thermo Fisher verso cdna kit
$(A) Expression of LmSLC26A at RNA level. Total RNA was extracted from L. major <t>cell,</t> <t>DNAse</t> treated and <t>cDNA</t> was prepared. The lanes marked as “+RT” and “−RT” represent the reactions with and without RT enzyme, respectively. (B) Topological schematic of LmSLC26A with Transmembrane domain, STAS domain, and Histidine phosphatase domain. The red line represents the region selected for antibody generation. (C) Purification of LmSLC26A 597-935 from bacterial expression system with Ni-NTA affinity purification. WCL- Whole cell lysate, IF- insoluble fraction, SF- soluble fraction, FT- flow through, W- Wash, E- Elute fraction (D) Generation of antibody in mice. Western blot was performed using purified protein with prebleed and test bleed. (E) Generation of antibody in the rabbit. Western blot was performed using purified protein with prebleed and test bleed.$
Verso Cdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/verso+cdna+kit/verso+cdna+kit/bio_rxiv__2025__07__16__665125-232-30-33
Average 90 stars, based on 1 article reviews

verso cdna kit - by Bioz Stars, 2026-10

90/100 stars

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1) Product Images from "Identification of a novel bicarbonate transporter critical for Leishmania virulence"

Article Title: Identification of a novel bicarbonate transporter critical for Leishmania virulence

Journal: bioRxiv

doi: 10.1101/2025.07.16.665125

$(A) Expression of LmSLC26A at RNA level. Total RNA was extracted from L. major cell, DNAse treated and cDNA was prepared. The lanes marked as “+RT” and “−RT” represent the reactions with and without RT enzyme, respectively. (B) Topological schematic of LmSLC26A with Transmembrane domain, STAS domain, and Histidine phosphatase domain. The red line represents the region selected for antibody generation. (C) Purification of LmSLC26A 597-935 from bacterial expression system with Ni-NTA affinity purification. WCL- Whole cell lysate, IF- insoluble fraction, SF- soluble fraction, FT- flow through, W- Wash, E- Elute fraction (D) Generation of antibody in mice. Western blot was performed using purified protein with prebleed and test bleed. (E) Generation of antibody in the rabbit. Western blot was performed using purified protein with prebleed and test bleed.$
Figure Legend Snippet: (A) Expression of LmSLC26A at RNA level. Total RNA was extracted from L. major cell, DNAse treated and cDNA was prepared. The lanes marked as “+RT” and “−RT” represent the reactions with and without RT enzyme, respectively. (B) Topological schematic of LmSLC26A with Transmembrane domain, STAS domain, and Histidine phosphatase domain. The red line represents the region selected for antibody generation. (C) Purification of LmSLC26A 597-935 from bacterial expression system with Ni-NTA affinity purification. WCL- Whole cell lysate, IF- insoluble fraction, SF- soluble fraction, FT- flow through, W- Wash, E- Elute fraction (D) Generation of antibody in mice. Western blot was performed using purified protein with prebleed and test bleed. (E) Generation of antibody in the rabbit. Western blot was performed using purified protein with prebleed and test bleed.

Techniques Used: Expressing, Purification, Affinity Purification, Western Blot

Real-time Polymerase Chain Reaction:

Article Title: Therapy-induced senescence of glioblastoma cells is determined by the p21 CIP1 -CDK1/2 axis and does not require activation of DREAM.
Article Snippet: Preparation of RNA, real-time qPCR, methylation specific PCR and RNA-Seq Total RNA was isolated using the NucleoSpin® RNA Kit from Macherey-Nagel (Düren, Germany). .. Utilizing the Verso cDNA Kit (Thermo Scientific, Dreieich, Germany), 1 μg total RNA was transcribed into cDNA and qPCR was performed in technical triplicates using the GoTaq® qPCR Master Mix Protocol (Promega, Madison, USA) and the CFX96 Real-Time PCR Detection System (Biorad, München, Germany). ..

Article Title: Therapy-induced senescence of glioblastoma cells is determined by the p21 CIP1 -CDK1/2 axis and does not require activation of DREAM
Article Snippet: Total RNA was isolated using the NucleoSpin ® RNA Kit from Macherey-Nagel (Düren, Germany). .. Utilizing the Verso cDNA Kit (Thermo Scientific, Dreieich, Germany), 1 μg total RNA was transcribed into cDNA and qPCR was performed in technical triplicates using the GoTaq ® qPCR Master Mix Protocol (Promega, Madison, USA) and the CFX96 Real-Time PCR Detection System (Biorad, München, Germany). ..

Reverse Transcription:

Article Title: Unveiling the hidden threat: Molecular, cellular and behavioral effects of dietborne inorganic mercury and methylmercury in Dreissena polymorpha.
Article Snippet: .. Reverse transcription (RT) was performed using 400 ng of RNA with the Thermo Scientific Verso cDNA kit employing a mixture of oligo-dT and random primers as specified in manufacturer instructions. .. Quantitative PCR (qPCR) analysis was conducted using a BioRad CFX 96 system with Absolute Blue SYBR Green ROX Mix (Thermo Scientific) on cDNA samples diluted (1 : 10) in a total volume of 15 μL.

Article Title: Integrative Wound-Healing Effects of Clinacanthus nutans Extract and Schaftoside Through Anti-Inflammatory, Endothelial-Protective, and Antiviral Mechanisms.
Article Snippet: .. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Total RNA was extracted using the Total RNA Kit (Omega Bio-Tek, Norcross, GA, USA) and reverse-transcribed into cDNA using the Verso cDNA Kit (Thermo Scientific), following the manufacturer’s instructions. .. PCR amplification was performed using ReadyMix Taq polymerase (Sigma-Aldrich), with specific primers targeting mouse COX-2, iNOS, IL-6, TNF-α, PGE2 (the EP2 receptor is a subtype of the PGE2 receptor family), and GAPDH, as listed in Table 1.

cDNA Synthesis:

Article Title: Advancing pancreatic cancer therapy by mesothelin-specific nanobody conjugation.
Article Snippet: Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis Total RNA was extracted from cells using the RNeasy Mini Kit (Qiagen). .. Complementary DNA (cDNA) synthesis was performed with the Verso cDNA kit (Thermo Scientific) using 50 ng of total RNA. .. The qPCR reactions were conducted in triplicate with Power SYBR Green PCR Master Mix (Applied Biosystems) on a QuantStudio 1 system (Applied Biosystems), employing specific primers listed in Table S1.

Software:

Article Title:
Article Snippet: Sigma-Aldrich Catalog no. NA931, RRID:AB_772210 (1:2000 dilution) pLenti-CmGFP-human CD5L vector OriGene, Rockville, MD Catalog no. RC206528L2 Critical commercial assays Click-iT EdU Assay Kit Invitrogen Catalog no. C10632 .. Kit Mybiosource, San Diego, CA, USA Catalog no. MBS2024653 Verso cDNA kit Thermo Scientific Catalog no. AB1453B RNeasy mini kit Qiagen Catalog no. 74106 EZ ChIPTM kit Millipore, Temecula, CA Catalog no. 17-371 Software and algorithms GraphPad Prism 7.0. .. GraphPad Prism 7.0.

Reverse Transcription Polymerase Chain Reaction:

Isolation:

Article Title: Identification of a novel bicarbonate transporter critical for Leishmania virulence
Article Snippet: .. Total RNA from L. major promastigotes was isolated using Trizol reagent (Invitrogen, Carlsbad, CA, USA), followed by DNase I treatment (Invitrogen). cDNA was prepared from DNase I-treated RNA using the Verso cDNA kit (Thermo Scientific, Waltham, MA, USA) using the manufacturer’s protocol. ..

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